Tumour Evolution

McPherson A, et al. Divergent Modes of Clonal Spread and Intraperitoneal Mixing in High-Grade Serous Ovarian Cancer. Nature Genetics. Published online May 16, 2016. doi:10.1038/ng.3573

Our Nature Genetics paper tracked the patterns of metastatic spread in the peritoneal cavity of patients with high grade serous ovarian cancer. we identify at least two divergent modes of intraperitoneal metastasis, highlighting the interplay between genomically diverse clones and their migratory potential prior to therapeutic intervention in high grade serous ovarian cancers.

 

Interactive visualizations of each patient:

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Patient 1       Patient 2       Patient 3       Patient 4       Patient 7       Patient 9       Patient 10

 

How to interact with the visualization:

The visualization has a main view consisting of a patient’s tumour samples centrally connected to an anatomical diagram. Hover over each anatomical location to highlight its associated tumour samples and to view its location name. Each sample may be reordered by clicking and dragging its title. There are two representations per sample: (1) a cell aggregate qualitatively displaying the prevalence of each clone, and (2) a phylogeny of clones participating in that tumour. Hover over a clone in the phylogeny to display the proportion of the sample belonging to that clone.

The legend begins with the patient’s overarching clonal phylogeny. Hover over a branch in the phylogeny to focus on that lineage. Similarly, hover over a clone in the phylogeny to focus on that clone, and additionally, to view its prevalence in each sample. Click on a clone to filter the mutation table such that it shows only those mutations originating in the selected clone. Note that the visualization supports the clicking of multiple clones. To exit a clone selection, click the reset button at the top left corner of the view. Next in the legend is the full anatomical diagram and the circled region of interest. Lastly, the legend contains a list of mixture classes present in the patient. Hover over these classes to highlight the corresponding tumour samples.

The mutation table houses the patient’s deep sequencing mutations. Use the Search box to filter mutations by chromosome, coordinate, gene, nucleotide change, etc. Click on a mutation to (1) highlight the legend phylogeny branch in which it occurred, (2) highlight the samples in which that mutation is present, and (3) display its variant allele frequency in each sample. To exit the mutation selection, click the reset button at the top left corner of the view.

To download an SVG or PNG of the visualization, click the appropriate button at the top right corner of the view.